Protein crystallization facilitated by molecularly imprinted polymers
نویسندگان
چکیده
منابع مشابه
Protein crystallization facilitated by molecularly imprinted polymers.
We present a previously undescribed initiative and its application, namely the design of molecularly imprinted polymers (MIPs) for producing protein crystals that are essential for determining high-resolution 3D structures of proteins. MIPs, also referred to as "smart materials," are made to contain cavities capable of rebinding protein; thus the fingerprint of the protein created on the polyme...
متن کاملSelective Extractions by Molecularly Imprinted Polymers (MIPs)
Sample preparation is often the rate-limiting step in analytical methods and its effectiveness has a critical impact on accuracy, quantification and, ultimately, the precision of any measurement. Traditionally, liquid–liquid extraction methods are frequently used for the extraction of organic analytes from aqueous samples. To overcome some of the disadvantages of liquid–liquid extraction (e.g.,...
متن کاملQuantification and confocal imaging of protein specific molecularly imprinted polymers.
We have employed FITC--albumin as the protein template molecule in an aqueous phase molecular imprinted polymer (HydroMIP) strategy. For the first time, the use of a fluorescently labeled template is reported, with subsequent characterization of the smart material to show that the HydroMIP possesses a significant molecular memory in comparison to that of the nonimprinted control polymer (HydroN...
متن کاملCitrinin selective molecularly imprinted polymers for SPE.
Molecularly imprinted polymers (MIPs) for citrinin (Cit) with 1-hydroxy-2-naphthoic acid (HNA) as mimic template were prepared and the molecularly imprinted SPE method was developed for the detection of Cit in rice with HPLC. The adsorption properties of HNA and Cit on the MIPs and nonimprinted polymers were investigated. It proved that MIPs showed higher selectivity adsorption to HNA and Cit t...
متن کاملMolecularly Imprinted Polymers for Sample Preparation
The sample treatment/preparation steps involve any operation and manipulation of the samples prior to instrumental determination of the target compounds [1−3]. This is a critical step in the analytical process because the concentration of target analytes in the samples is frequently too low for quantification. Thus, the first step of the preparation protocol involves the exhaustive extraction/c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2011
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1016539108